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pslik hygro shin  (Addgene inc)


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    Addgene inc pslik hygro shin
    Pslik Hygro Shin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pslik+hygro+shin/pSLIK-Hygro+(Plasmid+%2325737)/pm39178116-567-62-66
    Average 93 stars, based on 45 article reviews
    pslik hygro shin - by Bioz Stars, 2026-11
    93/100 stars

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    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER pLKO.1_Art1_shRNA_1 Dharmacon TRCN0000110341 pLKO.1_Art1_shRNA_2 Dharmacon TRCN0000110342 pLKO.1_Non-targeting_shRNA Dharmacon RHS6848 pEN_TTmcs Shin et al., 2006 Addgene # 25755 pSLIK_Hygro Shin et al., 2006 Addgene # 25737 pMDLg/pRRE Dull et al., 1998 Gift from Didier Trono, Addgene # 12251 pRSV-REV Dull et al., 1998 Gift from Didier Trono, Addgene # 12253 pCMV-VSV-G Dull et al., 1998 Gift from Didier Trono, Addgene # 8454 Software and algorithms R statistical programming environment https://www.r-project.org Version R 3.5.1 RMS (R-package) N/A Version 5.1-2 Limma (R-package) Ritchie et al., 2015 Version 3.36.3 STAR: RNA-Seq aligner Dobin et al., 2013 Version 2.5.0 HT-Seq Anders et al., 2015 Version 0.6.1 DeSeq2 (R-package) Love et al., 2014 Version 1.20.0 DAVID Bioinformatics https://david.ncifcrf.gov Version 6.8 Enrichr Kuleshov et al., 2016 N/A GeneOverlap (R-package) https://bioconductor.org/packages/ release/bioc/html/GeneOverlap.html Version 1.16.0 Figi (ImageJ) https://fiji.sc N/A Other Raw Microarray Data Liu et al., 2013 GEO: GSE47177 Raw RNA-Seq Data Ryall et al., 2015 GEO: GSE64379 Raw RNA-Seq Data Charville et al., 2015 ENA: PRJEB10091 Processed H3K27ac ChIP-Seq Data Machado et al., 2017 GEO: GSE103163 Article ll OPEN ACCESS ..

    Article Title: CRISPR screening identifies PRMT1 as a key pro-ferroptotic gene via a two-layer regulatory mechanism.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DAPI Sigma-Aldrich Cat#D9542 ECL UltraPlus Western HRP Substrate Millipore Cat#WBULP glutathione Sepharose 4B beads Cytiva Cat#17075601 S-(50-Adenosyl)-L-methionine chloride (SAM) Cayman Cat#13956 Anti-Flag M2 affinity gel Sigma-Aldrich Cat#A2220 Anti-V5 affinity gel Abclonal Cat#AE029 Protein A/G agarose beads Proteintech Cat#PR40025 doxycycline MedChemExpress Cat#HY-N0565A Biotin MedChemExpress Cat#HY-B0511 DynabeadsTM MyOneTM Streptavidin T1 bead slurry Thermo Cat#65601 alkynyl myristic acid analogue (YnMyr) MedChemExpress Cat#HY-140335 TAMRA-Azide-PEG-biotin MedChemExpress Cat#HY-140497 tris(2-carboxyethyl)phosphine (TCEP) MedChemExpress Cat#HY-W011500 tris(benzyltriazolylmethyl)amine (TBTA) MedChemExpress Cat#HY-116677 CuSO4 Sigma-Aldrich Cat#451657 Critical commercial assays LDH Cytotoxicity Assay Kit Beyotime Cat#C0016 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M GSH and GSSG Assay Kit Beyotime Cat#S0053 Membrane and Cytosol Protein Extraction Kit Beyotime Cat#P0033 b-galactosidase Assay Kit Beyotime Cat#RG0036 reduced CoQ10 ELISA kit Jianglai Bio Cat#JL50742 Hifair III 1st Strand cDNA Synthesis SuperMix Yeasen Cat#11141ES60 Eastep qPCR Master Mix kit Promega Cat#LS2062 QIAamp DNA Blood Midi Kit QIAGEN Cat#51183 luciferase reporter assay kit Promega Cat#E1501 EZ-Magna ChIP A/G Chromatin Immunoprecipitation Kit Millipore Cat#17-10086 Aspartate Aminotransferase (AST) Activity Assay Kit Elabscience Cat#E-BC-K236-M Alanine Aminotransferase (ALT) Activity Assay Kit Elabscience Cat#E-BC-K235-M SignalStain DAB Substrate Kit Cell Signaling Cat#8059 MycoBlue Mycoplasma Detector Kit Vazyme Cat#D101 Deposited data RNA-seq data of Prmt1 KO cells This paper GSA: CRA017505 The mass spectrometry proteomics data This paper PRIDE: PXD054515 Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 B16F10 ATCC Cat#CRL-6475 Hepa1-6 ATCC Cat#CRL-1830 HT1080 ATCC Cat#CCL-121 U87MG ATCC Cat#HTB-14 Experimental models: Organisms/strains Mouse: C57BL/6 GemPharmatech Company N/A Oligonucleotides sgRNA/shRNA/siRNA sequences are listed in Table S2 This paper N/A Primers for qPCR are listed in Table S3 This paper N/A Primers for ChIP are listed in Table S3 This paper N/A Recombinant DNA PRMT1-V5 This paper N/A (Continued on next page) Cell Reports 43, 114662, September 24, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER PRMT1(E153Q)-V5 This paper N/A PRMT1-Flag This paper N/A FSP1-Flag This paper N/A FSP1-V5 This paper N/A FSP1(R316K)-V5 This paper N/A FSP1(G2A)-V5 This paper N/A GSP1(G2A/R316K)-V5 This paper N/A GST-FSP1 This paper N/A GST-FSP1(R316K) This paper N/A His-PRMT1 This paper N/A mCherry-Golgi This paper N/A pcDNA3.1 myc-BioID2 Kim et al.29 Addgene Cat#74223 pEN_TTmcs Shin et al.69 Addgene Cat#25755 pSLIK-Hygro Shin et al.69 Addgene Cat25737 pEN_TT-myc-BioID2-MCS This paper N/A pEN_TT-myc-BioID2-PRMT1 This paper N/A pSLIK-myc-BioID2-PRMT1 This paper N/A pGL3-SLC7A11 promoter This paper N/A lentiCRISPR-sgRNAs for human or mouse genes, see Table S2 This paper N/A pLKO-shRNAs for human or mouse genes, see Table S2 This paper N/A lentiCas9-Blast Sanjana et al.70 Addgene Cat#52962 psPAX2 a gift from Didier Trono Addgene Cat#12260 pMD2.G a gift from Didier Trono Addgene Cat#12259 Software and algorithms GraphPad Prism 9 GraphPad Software Inc. RRID: SCR_002798 Flowjo10 Becton Dickinson & Company RRID: SCR_008520 cellSens Entry software OLYMPUS RRID:SCR_014551 ..

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLKO.1_Art1_shRNA_1 Dharmacon TRCN0000110341 pLKO.1_Art1_shRNA_2 Dharmacon TRCN0000110342 pLKO.1_Non-targeting_shRNA Dharmacon RHS6848 pEN_TTmcs Shin et al., 2006 Addgene # 25755 pSLIK_Hygro Shin et al., 2006 Addgene # 25737 pMDLg/pRRE Dull et al., 1998 Gift from Didier Trono, Addgene # 12251 pRSV-REV Dull et al., 1998 Gift from Didier Trono, Addgene # 12253 pCMV-VSV-G Dull et al., 1998 Gift from Didier Trono, Addgene # 8454 Software and algorithms R statistical programming environment https://www.r-project.org Version R 3.5.1 RMS (R-package) N/A Version 5.1-2 Limma (R-package) Ritchie et al., 2015 Version 3.36.3 STAR: RNA-Seq aligner Dobin et al., 2013 Version 2.5.0 HT-Seq Anders et al., 2015 Version 0.6.1 DeSeq2 (R-package) Love et al., 2014 Version 1.20.0 DAVID Bioinformatics https://david.ncifcrf.gov Version 6.8 Enrichr Kuleshov et al., 2016 N/A GeneOverlap (R-package) https://bioconductor.org/packages/ release/bioc/html/GeneOverlap.html Version 1.16.0 Figi (ImageJ) https://fiji.sc N/A Other Raw Microarray Data Liu et al., 2013 GEO: GSE47177 Raw RNA-Seq Data Ryall et al., 2015 GEO: GSE64379 Raw RNA-Seq Data Charville et al., 2015 ENA: PRJEB10091 Processed H3K27ac ChIP-Seq Data Machado et al., 2017 GEO: GSE103163 ..

    RNA sequencing:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER pLKO.1_Art1_shRNA_1 Dharmacon TRCN0000110341 pLKO.1_Art1_shRNA_2 Dharmacon TRCN0000110342 pLKO.1_Non-targeting_shRNA Dharmacon RHS6848 pEN_TTmcs Shin et al., 2006 Addgene # 25755 pSLIK_Hygro Shin et al., 2006 Addgene # 25737 pMDLg/pRRE Dull et al., 1998 Gift from Didier Trono, Addgene # 12251 pRSV-REV Dull et al., 1998 Gift from Didier Trono, Addgene # 12253 pCMV-VSV-G Dull et al., 1998 Gift from Didier Trono, Addgene # 8454 Software and algorithms R statistical programming environment https://www.r-project.org Version R 3.5.1 RMS (R-package) N/A Version 5.1-2 Limma (R-package) Ritchie et al., 2015 Version 3.36.3 STAR: RNA-Seq aligner Dobin et al., 2013 Version 2.5.0 HT-Seq Anders et al., 2015 Version 0.6.1 DeSeq2 (R-package) Love et al., 2014 Version 1.20.0 DAVID Bioinformatics https://david.ncifcrf.gov Version 6.8 Enrichr Kuleshov et al., 2016 N/A GeneOverlap (R-package) https://bioconductor.org/packages/ release/bioc/html/GeneOverlap.html Version 1.16.0 Figi (ImageJ) https://fiji.sc N/A Other Raw Microarray Data Liu et al., 2013 GEO: GSE47177 Raw RNA-Seq Data Ryall et al., 2015 GEO: GSE64379 Raw RNA-Seq Data Charville et al., 2015 ENA: PRJEB10091 Processed H3K27ac ChIP-Seq Data Machado et al., 2017 GEO: GSE103163 Article ll OPEN ACCESS ..

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLKO.1_Art1_shRNA_1 Dharmacon TRCN0000110341 pLKO.1_Art1_shRNA_2 Dharmacon TRCN0000110342 pLKO.1_Non-targeting_shRNA Dharmacon RHS6848 pEN_TTmcs Shin et al., 2006 Addgene # 25755 pSLIK_Hygro Shin et al., 2006 Addgene # 25737 pMDLg/pRRE Dull et al., 1998 Gift from Didier Trono, Addgene # 12251 pRSV-REV Dull et al., 1998 Gift from Didier Trono, Addgene # 12253 pCMV-VSV-G Dull et al., 1998 Gift from Didier Trono, Addgene # 8454 Software and algorithms R statistical programming environment https://www.r-project.org Version R 3.5.1 RMS (R-package) N/A Version 5.1-2 Limma (R-package) Ritchie et al., 2015 Version 3.36.3 STAR: RNA-Seq aligner Dobin et al., 2013 Version 2.5.0 HT-Seq Anders et al., 2015 Version 0.6.1 DeSeq2 (R-package) Love et al., 2014 Version 1.20.0 DAVID Bioinformatics https://david.ncifcrf.gov Version 6.8 Enrichr Kuleshov et al., 2016 N/A GeneOverlap (R-package) https://bioconductor.org/packages/ release/bioc/html/GeneOverlap.html Version 1.16.0 Figi (ImageJ) https://fiji.sc N/A Other Raw Microarray Data Liu et al., 2013 GEO: GSE47177 Raw RNA-Seq Data Ryall et al., 2015 GEO: GSE64379 Raw RNA-Seq Data Charville et al., 2015 ENA: PRJEB10091 Processed H3K27ac ChIP-Seq Data Machado et al., 2017 GEO: GSE103163 ..

    Microarray:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER pLKO.1_Art1_shRNA_1 Dharmacon TRCN0000110341 pLKO.1_Art1_shRNA_2 Dharmacon TRCN0000110342 pLKO.1_Non-targeting_shRNA Dharmacon RHS6848 pEN_TTmcs Shin et al., 2006 Addgene # 25755 pSLIK_Hygro Shin et al., 2006 Addgene # 25737 pMDLg/pRRE Dull et al., 1998 Gift from Didier Trono, Addgene # 12251 pRSV-REV Dull et al., 1998 Gift from Didier Trono, Addgene # 12253 pCMV-VSV-G Dull et al., 1998 Gift from Didier Trono, Addgene # 8454 Software and algorithms R statistical programming environment https://www.r-project.org Version R 3.5.1 RMS (R-package) N/A Version 5.1-2 Limma (R-package) Ritchie et al., 2015 Version 3.36.3 STAR: RNA-Seq aligner Dobin et al., 2013 Version 2.5.0 HT-Seq Anders et al., 2015 Version 0.6.1 DeSeq2 (R-package) Love et al., 2014 Version 1.20.0 DAVID Bioinformatics https://david.ncifcrf.gov Version 6.8 Enrichr Kuleshov et al., 2016 N/A GeneOverlap (R-package) https://bioconductor.org/packages/ release/bioc/html/GeneOverlap.html Version 1.16.0 Figi (ImageJ) https://fiji.sc N/A Other Raw Microarray Data Liu et al., 2013 GEO: GSE47177 Raw RNA-Seq Data Ryall et al., 2015 GEO: GSE64379 Raw RNA-Seq Data Charville et al., 2015 ENA: PRJEB10091 Processed H3K27ac ChIP-Seq Data Machado et al., 2017 GEO: GSE103163 Article ll OPEN ACCESS ..

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLKO.1_Art1_shRNA_1 Dharmacon TRCN0000110341 pLKO.1_Art1_shRNA_2 Dharmacon TRCN0000110342 pLKO.1_Non-targeting_shRNA Dharmacon RHS6848 pEN_TTmcs Shin et al., 2006 Addgene # 25755 pSLIK_Hygro Shin et al., 2006 Addgene # 25737 pMDLg/pRRE Dull et al., 1998 Gift from Didier Trono, Addgene # 12251 pRSV-REV Dull et al., 1998 Gift from Didier Trono, Addgene # 12253 pCMV-VSV-G Dull et al., 1998 Gift from Didier Trono, Addgene # 8454 Software and algorithms R statistical programming environment https://www.r-project.org Version R 3.5.1 RMS (R-package) N/A Version 5.1-2 Limma (R-package) Ritchie et al., 2015 Version 3.36.3 STAR: RNA-Seq aligner Dobin et al., 2013 Version 2.5.0 HT-Seq Anders et al., 2015 Version 0.6.1 DeSeq2 (R-package) Love et al., 2014 Version 1.20.0 DAVID Bioinformatics https://david.ncifcrf.gov Version 6.8 Enrichr Kuleshov et al., 2016 N/A GeneOverlap (R-package) https://bioconductor.org/packages/ release/bioc/html/GeneOverlap.html Version 1.16.0 Figi (ImageJ) https://fiji.sc N/A Other Raw Microarray Data Liu et al., 2013 GEO: GSE47177 Raw RNA-Seq Data Ryall et al., 2015 GEO: GSE64379 Raw RNA-Seq Data Charville et al., 2015 ENA: PRJEB10091 Processed H3K27ac ChIP-Seq Data Machado et al., 2017 GEO: GSE103163 ..

    Chromatin Immunoprecipitation:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER pLKO.1_Art1_shRNA_1 Dharmacon TRCN0000110341 pLKO.1_Art1_shRNA_2 Dharmacon TRCN0000110342 pLKO.1_Non-targeting_shRNA Dharmacon RHS6848 pEN_TTmcs Shin et al., 2006 Addgene # 25755 pSLIK_Hygro Shin et al., 2006 Addgene # 25737 pMDLg/pRRE Dull et al., 1998 Gift from Didier Trono, Addgene # 12251 pRSV-REV Dull et al., 1998 Gift from Didier Trono, Addgene # 12253 pCMV-VSV-G Dull et al., 1998 Gift from Didier Trono, Addgene # 8454 Software and algorithms R statistical programming environment https://www.r-project.org Version R 3.5.1 RMS (R-package) N/A Version 5.1-2 Limma (R-package) Ritchie et al., 2015 Version 3.36.3 STAR: RNA-Seq aligner Dobin et al., 2013 Version 2.5.0 HT-Seq Anders et al., 2015 Version 0.6.1 DeSeq2 (R-package) Love et al., 2014 Version 1.20.0 DAVID Bioinformatics https://david.ncifcrf.gov Version 6.8 Enrichr Kuleshov et al., 2016 N/A GeneOverlap (R-package) https://bioconductor.org/packages/ release/bioc/html/GeneOverlap.html Version 1.16.0 Figi (ImageJ) https://fiji.sc N/A Other Raw Microarray Data Liu et al., 2013 GEO: GSE47177 Raw RNA-Seq Data Ryall et al., 2015 GEO: GSE64379 Raw RNA-Seq Data Charville et al., 2015 ENA: PRJEB10091 Processed H3K27ac ChIP-Seq Data Machado et al., 2017 GEO: GSE103163 Article ll OPEN ACCESS ..

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLKO.1_Art1_shRNA_1 Dharmacon TRCN0000110341 pLKO.1_Art1_shRNA_2 Dharmacon TRCN0000110342 pLKO.1_Non-targeting_shRNA Dharmacon RHS6848 pEN_TTmcs Shin et al., 2006 Addgene # 25755 pSLIK_Hygro Shin et al., 2006 Addgene # 25737 pMDLg/pRRE Dull et al., 1998 Gift from Didier Trono, Addgene # 12251 pRSV-REV Dull et al., 1998 Gift from Didier Trono, Addgene # 12253 pCMV-VSV-G Dull et al., 1998 Gift from Didier Trono, Addgene # 8454 Software and algorithms R statistical programming environment https://www.r-project.org Version R 3.5.1 RMS (R-package) N/A Version 5.1-2 Limma (R-package) Ritchie et al., 2015 Version 3.36.3 STAR: RNA-Seq aligner Dobin et al., 2013 Version 2.5.0 HT-Seq Anders et al., 2015 Version 0.6.1 DeSeq2 (R-package) Love et al., 2014 Version 1.20.0 DAVID Bioinformatics https://david.ncifcrf.gov Version 6.8 Enrichr Kuleshov et al., 2016 N/A GeneOverlap (R-package) https://bioconductor.org/packages/ release/bioc/html/GeneOverlap.html Version 1.16.0 Figi (ImageJ) https://fiji.sc N/A Other Raw Microarray Data Liu et al., 2013 GEO: GSE47177 Raw RNA-Seq Data Ryall et al., 2015 GEO: GSE64379 Raw RNA-Seq Data Charville et al., 2015 ENA: PRJEB10091 Processed H3K27ac ChIP-Seq Data Machado et al., 2017 GEO: GSE103163 ..



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